Alberta Amu Quartey

University

Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

PhD Title

Estimation 0f Malaria Transmission Intensity in Southern Ghana Using RDT Derived Seroprevalence Rates

Abstract

Malaria Control measures are evaluated using Malaria transmission. Entomological studies are the gold standard, but logistical and financial costs are taxing. Studies have validated the ease and accuracy of using serological surveys to estimate malaria transmission using Rapid Diagnostic Tests (RDTs).

Objective: to estimate malaria transmission using sero-prevalence rates in the Dangme West District.

Hypothesis: Malaria sero-conversion rates for the same exposure differ according to age.

Methods: District population: 108,344, with three zones, coastal, lakeside and forest. A three part study using random participant selection with equal zonal allocation;

Prevalence: Two surveys timed to coincide with dry and wet seasons endings, covers 2550 participants, History of fever (last 2 weeks) ACT and bednets use. Finger blood samples will be taken (RDT, thick and thin blood films.)

Used RDT cassettes, stored below 4°C will be processed to elute sera for IgG recognizing merozoite surface protein-119 (MSP-119) using indirect ELISA.

Cohort: 1635 participants will be visited monthly (twelve rounds) and history of fever in the past 2 weeks sought and investigated.

Entomology: Mosquito collections will be done by Human Landing and Pyrethrum Spray Catches from four randomly selected houses per month per zone

Outcomes: Zonal parasite prevalence, Malaria incidence, MSP-119 prevalence, EIRs